Chemical analysis of a sulphated macromolecule isolated from rabbit submandibular gland

Chemical analysis of a sulphated macromolecule isolated from the rabbit submandibular gland showed that about 70 per cent of the dry weight could be accounted for in terms of amino acids of which the acidic amino acids, proline and alanine were the most abundant. Total carbohydrate was 11%. Galactose was the main neutral sugar but galactosamine, glucosamine, neuraminic acid, glucose and mannose could also be demonstrated. Sulphate accounted for 1,4%. No uronic acid was found. The data indicate that the sulphated macromolecule from the rabbit gland is in fact a sulphated glycoprotein.