Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Non-unity molecular heritability demonstrated by continuous evolution in vitro.

T Schmitt1, N Lehman

  • 1Department of Biological Sciences, University at Albany, NY 12222, USA.

Chemistry & Biology
|January 13, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robotic-assisted bilateral lumbar pars fracture endoscopic debridement and direct repair as treatment for lumbar radiculopathy: A case report.

North American Spine Society journal·2025
Same author

Coexistence of Insulatorlike Paramagnon and Metallic Spin-Orbit Exciton Modes in SrIrO_{3}.

Physical review letters·2025
Same author

Poly(vinyl alcohol) cryogels: Effect size of polymer concentration, number of cycles and thawing rate on material properties and dermal drug delivery.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2025
Same author

Magnon interactions in a moderately correlated Mott insulator.

Nature communications·2024
Same author

Fate of charge order in overdoped La-based cuprates.

npj quantum materials·2024
Same author

Author Correction: Universal DNA methylation age across mammalian tissues.

Nature aging·2023

In vitro evolution selected a ribozyme variant that avoids misfolding into inactive states. This demonstrates how molecular systems can evolve to favor specific phenotypes, suggesting life-like characteristics in RNA.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Evolutionary Biology

Background:

  • Catalytic RNA (ribozyme) evolution typically targets chemical reactivity.
  • Non-unity heritability, where one genotype yields multiple phenotypes, complicates RNA evolution.
  • This study investigates non-unity heritability's influence on ribozyme evolution.

Purpose of the Study:

  • To test the impact of non-unity heritability on molecular evolution.
  • To understand how RNA molecules evolve to favor specific functional states.

Main Methods:

  • Continuous evolution of a ligase ribozyme over 20 bursts.
  • Periodic reduction of magnesium (Mg2+) concentration during evolution.
  • Kinetic analyses and conformational examination of evolved variants.
Keywords:
NASA Discipline ExobiologyNon-NASA Center

Related Experiment Videos

Main Results:

  • A nine-error variant became dominant, showing increased activity.
  • The variant's advantage stems from reduced misfolding into inactive conformations, not a higher catalytic rate.
  • Multiple RNA conformations were observed under evolving conditions.

Conclusions:

  • Genotypes evolving to favor specific phenotypes can occur at the molecular level.
  • In vitro RNA evolution exhibits life-like characteristics.
  • Findings support the RNA world hypothesis.