Related Experiment Video
Updated: Aug 19, 2026

08:13
Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes
Published on: September 1, 2018
Increased sensitivity to oxidative challenges associated with topA deletion in Escherichia coli
1Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA. yuk-ching_tse-dinh@nymc.edu
Journal of Bacteriology
|January 14, 2000
Abstract:
Deletion of topA in Escherichia coli was found to result in a higher level of killing after treatment with either hydrogen peroxide or N-ethylmaleimide. This effect on oxidative challenge response represents a new role for E. coli DNA topoisomerase I in addition to prevention of excessive negative supercoiling of DNA.

