Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Laser-assisted microdissection and molecular analysis at the cellular level].

M R Bernsen1, G N van Muijen, D J Ruiter

  • 1Academisch Ziekenhuis St. Radboud, afd. Pathologie, Nijmegen.

Nederlands Tijdschrift Voor Geneeskunde
|January 14, 2000
PubMed
Summary

Laser-assisted microdissection enables isolating pure cell populations from tissues for molecular analysis. This technique improves accuracy in studying disease genetics by linking morphology to molecular data.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SPECT/CT imaging of inflammation and calcification in human carotid atherosclerosis to identify the plaque at risk of rupture.

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2021
Same author

Perspectives on Small Animal Radionuclide Imaging; Considerations and Advances in Atherosclerosis.

Frontiers in medicine·2019
Same author

Mesenchymal stem cell secretome reduces pain and prevents cartilage damage in a murine osteoarthritis model.

European cells & materials·2018
Same author

Imaging of atherosclerosis, targeting LFA-1 on inflammatory cells with <sup>111</sup>In-DANBIRT.

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2018
Same author

Radiological imaging of teratological fetuses: what can we learn?

Insights into imaging·2017
Same author

Encapsulation of allogeneic mesenchymal stem cells in alginate extends local presence and therapeutic function.

European cells & materials·2017

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Advancements in molecular biology allow analysis of small tissue samples.
  • Obtaining pure cell populations is crucial for reliable molecular analysis but remains a challenge.
  • Pure cell samples are essential for accurate biomedical research and disease studies.

Purpose of the Study:

  • To highlight the utility of laser-assisted microdissection for isolating pure cell populations.
  • To explain how this technique facilitates molecular genetic studies of diseases.
  • To discuss the future potential of laser microdissection in biomedical research.

Main Methods:

  • Utilizing laser-assisted microdissection systems to isolate pure cell populations from tissue sections.
  • Enabling isolation at the single-cell level.

Related Experiment Videos

  • Ensuring isolated cells are suitable for subsequent molecular analyses.

    • Main Results:

    • Laser-assisted microdissection allows the procurement of pure cell populations from tissue sections.
    • The technique is effective even at a single-cell level.
    • Morphological features can be directly correlated with molecular characteristics, enhancing study accuracy.

    Conclusions:

    • Laser-assisted microdissection overcomes limitations in obtaining pure cell samples for research.
    • It facilitates more accurate studies of the molecular genetic basis of diseases.
    • Future integration with new molecular analysis methods will expand its applications.