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Related Experiment Videos

Bone marrow processing for transplantation using the COBE spectra cell separator.

Z Korístek1, J Mayer

  • 1Department of Internal Medicine-Hematooncology, Masaryk University Hospital, Brno, Czech Republic.

Journal of Hematotherapy & Stem Cell Research
|January 14, 2000
PubMed
Summary
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The COBE Spectra cell separator effectively processes bone marrow (BM) for allogeneic bone marrow transplantation (BMT), reducing red blood cells (RBC) and preserving vital cells. Further investigation is needed to understand the wide recovery range of CD34+ cells and colony-forming units-granulocyte-macrophage (CFU-GM).

Area of Science:

  • Hematology
  • Transplantation Medicine
  • Cellular Therapy

Background:

  • Bone marrow (BM) processing is crucial for allogeneic bone marrow transplantation (BMT), aiming to reduce side effects and overcome ABO incompatibility.
  • Key considerations include volume reduction, and depletion of granulocytes and erythrocytes.

Purpose of the Study:

  • To evaluate the efficacy and safety of the COBE Spectra cell separator for BM processing in patients with hematologic malignancy and solid tumors.
  • To maximize the recovery of mononuclear cells (MNC) during BM processing.

Main Methods:

  • The COBE Spectra cell separator was used to process 42 BM harvests from 33 patients undergoing allogeneic BMT.
  • BM was collected from the posterior iliac crest under general anesthesia.
  • Key cell populations, including total nucleated cells (TNC), granulocytes, MNC, CD34+ cells, and colony-forming units-granulocyte-macrophage (CFU-GM), were quantified before and after processing.

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Main Results:

  • The processing achieved a mean removal of 98.2% of red blood cells (RBC).
  • Mean recovery rates included 35.8% TNC, 22.3% granulocytes, 78.8% MNC, 77.2% CD34+ cells, and 153.3% CFU-GM.
  • The process was noted to be fast, safe, and effective, with a mean of 13.3 ml of RBC remaining in the final product.

Conclusions:

  • BM processing with the COBE Spectra cell separator is a viable method for allogeneic BMT preparation.
  • The device effectively reduces RBC and preserves important cellular components.
  • The significant variability in CD34+ cell and CFU-GM recovery warrants further investigation.