Computer-vision-based extraction of neural dendrograms

This work introduces a new approach to the characterization of neural cells by means of semi-automated generation of dendrograms; data structures which describe the inherently hierarchical nature of neuronal arborizations. Dendrograms describe the branched structure of neurons in terms of the length, average thickness and bending energy of each of the dendritic segments and allow in a straightforward manner, the inclusion of additional measures. The bending energy quantifies the complexity of the shape and can be used to characterize the spatial coverage of the arborizations (the bending energy is an alternative for other complexity measures such as the fractal dimension). The new approach is based on the partitioning of the cell's outer contour as a function of the high curvature points followed by a syntactical analysis of the segmented contours. The semi-automated method is robust and is an improvement on the time consuming manual generation of the dendrograms. Several experimental results are included in this paper which illustrate and corroborate the effectiveness of the approach. The technique presented in this paper is limited to planar neurons but could be extended to a 3D approach.