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Distribution of ascorbate in the anterior bovine eye
A Ringvold1, E Anderssen, I Kjønniksen
1The National Hospital Pharmacy, Oslo, Norway. a.d.ringvold@klinmed.uio.no
Investigative Ophthalmology & Visual Science
|January 14, 2000
Summary
Ascorbic acid, an important antioxidant, is most concentrated in the central corneal epithelium, suggesting a role in UV protection. Aqueous humor aids in distributing this compound to the anterior eye wall.
Area of Science:
- Ophthalmology
- Biochemistry
- Nutritional Science
Background:
- Ascorbate (vitamin C) is a vital antioxidant with known roles in ocular health.
- Its distribution within the anterior segment of the eye is crucial for understanding its protective functions.
Purpose of the Study:
- To quantify ascorbate distribution across various tissues of the anterior bovine eye wall.
- To elucidate the functional significance of ascorbate in protecting ocular structures.
Main Methods:
- High-performance liquid chromatography (HPLC) was employed for precise ascorbate determination.
- Bovine eye tissues, including cornea, sclera, conjunctiva, lacrimal gland, and aqueous humor, were analyzed.
Main Results:
- The highest ascorbate concentration was found in the central corneal epithelium.
- Corneal stroma, Descemet's membrane/endothelium, and aqueous humor exhibited similar ascorbate levels.
- Sclera, conjunctiva, lacrimal gland, and serum showed significantly lower ascorbate concentrations.
Conclusions:
- The high concentration in the central corneal epithelium supports its role as a UV filter, protecting internal eye structures.
- The similar ascorbate levels in corneal stroma, DM/endothelium, and aqueous humor suggest the aqueous humor is key for anterior eye wall ascorbate distribution.