Related Experiment Video
Updated: Aug 11, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Regulation of abscisic acid-induced stomatal closure and anion channels by guard cell AAPK kinase
1Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA.
Abstract:
Abscisic acid (ABA) stimulates stomatal closure and thus supports water conservation by plants during drought. Mass spectrometry-generated peptide sequence information was used to clone a Vicia faba complementary DNA, AAPK, encoding a guard cell-specific ABA-activated serine-threonine protein kinase (AAPK). Expression in transformed guard cells of AAPK altered by one amino acid (lysine 43 to alanine 43) renders stomata insensitive to ABA-induced closure by eliminating ABA activation of plasma membrane anion channels. This information should allow cell-specific, targeted biotechnological manipulation of crop water status.
Related Concept Videos
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
Regulation of Transpiration by Stomata
Adaptations that Reduce Water Loss
Cell Signaling in Plants
GPCRs Regulate Adenylyl Cylase Activity
Two...
cAMP-dependent Protein Kinase Pathways

