Identification and assay of lamotrigine in human milk with gas chromatography and densitometry

Conditions necessary for isolation and assay of lamotrigine in the human milk with gas chromatography in the range of concentrations 2-20 micrograms/ml, and densitometry in the range of 2-10 micrograms/ml are discussed. Lamotrigine was extracted with methylene chloride from the alkaline solution. In gas chromatography capillary column DB-17, flame-ionizing detector, and octacozanole as the internal standard have been used. Densitometric measurements have been performed at 217 nm.