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Epimeric separation of L-ascorbic acid and D-isoascorbic acid by capillary zone electrophoresis
1Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan.
Journal of Agricultural and Food Chemistry
|January 19, 2000
Summary
Capillary zone electrophoresis effectively separates L-ascorbic acid (L-AA) and D-isoascorbic acid (D-IAA). Optimal separation was achieved using a 0.2 M borate buffer at pH 9.0 with 10% methanol.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- L-ascorbic acid (L-AA) and D-isoascorbic acid (D-IAA) are isomers with potential applications in food and pharmaceuticals.
- Efficient separation methods are crucial for quality control and analysis of these compounds.
Purpose of the Study:
- To develop and optimize capillary zone electrophoresis (CZE) conditions for the separation of L-AA and D-IAA.
- To investigate the impact of buffer concentration, pH, and methanol addition on separation efficiency.
Main Methods:
- Capillary zone electrophoresis (CZE) was employed using a fused silica capillary.
- Systematic variation of borate buffer concentration (0.05-0.25 M) and pH (7.5-9.0).
- Investigation of methanol concentration (0-10%) in the carrier electrolyte.
Main Results:
- Migration times for L-AA and D-IAA increased with rising pH.
- Resolution (Rs) of 12.98 was achieved at pH 9.0.
- Borate buffer concentrations above 0.1 M and methanol addition significantly enhanced separation, with optimal conditions at 0.2 M borate buffer (pH 9.0) and 10% methanol.
Conclusions:
- Capillary zone electrophoresis provides an effective method for separating L-AA and D-IAA.
- Optimized conditions involving specific buffer concentration, pH, and methanol content are crucial for high-resolution separation.