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Updated: Oct 18, 2026

Measurement of Nitric Oxide Production in Mouse Tracheal Epithelial Cell Cultures Differentiated at the Air Liquid Interface
Published on: July 3, 2026
The effect of air pollution on exhaled nitric oxide of atopic and nonatopic subjects
J G van Amsterdam1, A Hollander, J D Snelder
1Laboratory of Health Effects Research, Laboratory for Pathology and Immunobiology, Bilthoven, 3720 BA, The Netherlands.
Abstract:
Levels of exhaled nitric oxide (NO) were determined in well-characterized atopic and nonatopic subjects on 4 days with a different level of outdoor air pollution. The two groups matched well regarding spirometric values, i.e., no difference with regard to FEV(1), FVC, and peak flow. On the 4 test days asymptomatic atopic subjects exhaled 1.5- to 2.4-fold higher levels of NO compared with nonatopic subjects. In both groups the increase in exhaled NO in response to air pollution was similar (2.5 times maximal increase, P < 0.01). In conclusion, atopic subjects exhale higher levels of NO compared with nonatopic subjects, but respond to a similar degree to increased levels of air pollution.
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