Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelial no release caused by red wine polyphenols.

J C Stoclet1, A Kleschyov, E Andriambeloson

  • 1Pharmacologie et Physico-chimie des Interations Cellulaires et Moléculaires (UMR CNRS 7034), Université Louis Pasteur de Strasbourg, Illkirch, France.

Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society
|January 19, 2000
PubMed
Summary

Red wine polyphenolic compounds (RWPC) promote blood vessel relaxation by increasing nitric oxide (NO) production. Specific compounds like delphinidin are key, suggesting a mechanism for red wine

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Vascular dysfunction in obstructive sleep apnoea: Implication of microparticules].

Revue de pneumologie clinique·2017
Same author

[Impact of sickle cell trait on arterial stiffness in African subjects].

Journal de medecine vasculaire·2017
Same author

The selective 5-HT6 receptor antagonist SLV has putative cognitive- and social interaction enhancing properties in rodent models of cognitive impairment.

Neurobiology of learning and memory·2016
Same author

Microparticles as a means of cell-to-cell communication in endothelial dysfunction associated with type 1 diabetes.

Acta physiologica (Oxford, England)·2015
Same author

[Application to the rat of measurement of circulating blood volume and of the blood volume of the organs by erythrocytes labeled with Cr-51].

Comptes rendus des seances de la Societe de biologie et de ses filiales·2014
Same author

MRI measurement of liver fat content predicts the metabolic syndrome.

Diabetes & metabolism·2013

Area of Science:

  • Cardiovascular Pharmacology
  • Endothelial Cell Biology
  • Natural Product Chemistry

Background:

  • Epidemiological studies link moderate red wine consumption to reduced cardiovascular disease risk.
  • Red Wine Polyphenolic Compounds (RWPC) are complex extracts from red wine with potential health benefits.
  • Vascular function is crucial for cardiovascular health, with nitric oxide (NO) playing a key role.

Purpose of the Study:

  • To investigate the mechanism by which Red Wine Polyphenolic Compounds (RWPC) affect vascular function.
  • To determine if RWPC induce vasorelaxation and nitric oxide (NO) production in a controlled experimental setting.
  • To identify specific polyphenolic compounds responsible for the observed effects and their cellular targets.

Main Methods:

  • Rat aortic rings were used to assess endothelium-dependent vasorelaxation.

Related Experiment Videos

  • Nitric oxide (NO) formation was measured using electron paramagnetic resonance spectroscopy.
  • Intracellular calcium ([Ca2+]i) levels in endothelial and smooth muscle cells were analyzed.
  • Specific polyphenols were tested for their ability to mimic RWPC effects.

    • Main Results:

    • RWPC induced endothelium-dependent vasorelaxation in rat aortic rings.
    • This vasorelaxation was associated with significant NO formation, blocked by NO synthase inhibition.
    • Delphinidin, but not other tested polyphenols, mimicked the vasorelaxant effect of RWPC.
    • RWPC increased intracellular calcium ([Ca2+]i) in endothelial cells, but not smooth muscle cells, comparable to carbachol and bradykinin.

    Conclusions:

    • RWPC activate an undefined target in endothelial cells, leading to increased intracellular calcium.
    • This calcium increase likely activates NO-synthase, enhancing NO production and contributing to vasorelaxation.
    • These findings suggest a cellular mechanism for the cardiovascular protective effects associated with red wine polyphenols.