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Related Experiment Videos

Cutting edge: cytolytic effector function in human circulating CD8+ T cells closely correlates with CD56 surface

M J Pittet1, D E Speiser, D Valmori

  • 1Division of Clinical Onco-Immunology, Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland.

Journal of Immunology (Baltimore, Md. : 1950)
|January 21, 2000
PubMed
Summary

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Human effector CD8+ T cells, crucial for immunity, are best identified by CD56 expression. This marker, alongside CD8, precisely defines cytotoxic T lymphocyte (CTL) effector function and phenotype.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Human effector CD8+ T cells are critical for adaptive immunity.
  • Previous studies suggested a CD27-CD45RAhigh phenotype for these cells.

Purpose of the Study:

  • To investigate the role of CD56 (neuronal cell adhesion molecule) surface expression in defining human effector CD8+ T cell function.
  • To determine if CD56 is a more precise marker for cytotoxic T lymphocyte (CTL) effector function than previously identified markers.

Main Methods:

  • Flow cytometry analysis of peripheral blood CD8+ T cells from adult donors.
  • Assessment of intracellular perforin and granzyme B levels.
  • Evaluation of direct cytolytic capacity.

Main Results:

  • CD56 was expressed on a subset of circulating CD8+ T cells but absent on cord blood cells.

Related Experiment Videos

  • Oligoclonal expansions and high perforin/granzyme B levels were observed in the CD56+ subset.
  • Direct cytolytic capacity was predominantly found in CD56+ (CD45RAhigh) cells, outperforming the CD27-CD45RAhigh phenotype.

    • Conclusions:

    • CD56 surface expression is a more accurate and simplified marker for identifying circulating effector CD8+ T cells and their cytolytic function.
    • The phenotype of effector CD8+ T cells can be precisely defined using CD8 and CD56 markers.