Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Doping control for beta-adrenergic compounds through hair analysis.

P Kintz1, V Dumestre-Toulet, C Jamey

  • 1Institut de Médecine Légale, Strasbourg, France.

Journal of Forensic Sciences
|January 21, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A deadly root and the science behind it: LC-HRMS and LC-MS/MS analysis in an aconite-induced suicide.

Forensic toxicology·2025
Same author

Suitability of Hair Testing in Medical Dispensing Errors: A Fatal Case of Gliclazide Poisoning.

Drug testing and analysis·2024
Same author

Trends in fatal poisoning among medical users of analgesics in France from 2013 to 2022: an analysis of the DTA register.

Public health·2024
Same author

Semaglutide and health risk: Development and validation of a LC-HRMS method for testing semaglutide in whole blood and application to real cases.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2024
Same author

Bariatric surgery and changes in metformin distribution: About a fatal case.

Journal of pharmaceutical and biomedical analysis·2024
Same author

European Council of Legal Medicine (ECLM) on-site inspection forms for forensic pathology, anthropology, odontology, genetics, entomology and toxicology for forensic and medico-legal scene and corpse investigation: the Parma form.

International journal of legal medicine·2022

A new method accurately detects beta2-agonists like salbutamol and clenbuterol, and beta-blockers in hair. This hair analysis technique confirms drug use in athletes and animals.

Area of Science:

  • Forensic Toxicology
  • Analytical Chemistry

Background:

  • Detection of beta2-agonists and beta-blockers is crucial for doping control and clinical monitoring.
  • Existing methods for drug detection in hair can be limited in scope or sensitivity.

Purpose of the Study:

  • To develop and validate a novel analytical procedure for the simultaneous detection of specific beta2-agonists and beta-blockers in human and animal hair.
  • To establish a sensitive and reliable method for hair drug testing.

Main Methods:

  • Developed a procedure involving hair pulverization, overnight incubation in HCl, solid-phase extraction, and derivatization.
  • Utilized gas chromatography-mass spectrometry (GC/MS) for drug detection.
  • Validated linearity, limits of detection, and recovery rates for the tested compounds.

Related Experiment Videos

Main Results:

  • The method demonstrated linearity from 25 pg/mg to 10 ng/mg with low detection limits (2-10 pg/mg).
  • Successful application examples include detecting salbutamol in asthmatic patients, confirming salbutamol exposure in an athlete, and identifying chronic metoprolol and sotalol use in athletes.
  • The method also detected clenbuterol in animal hair, indicating its use for mass enhancement.

Conclusions:

  • The developed GC/MS method provides a reliable and sensitive approach for simultaneous analysis of beta2-agonists and beta-blockers in hair.
  • This technique has significant applications in forensic toxicology, anti-doping efforts, and veterinary drug monitoring.