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Mechanism of assembly of recombinant murine polyomavirus-like particles
1Institut f]ur Biotechnologie, Martin-Luther-Universit]at Halle-Wittenberg, D-06120 Halle (Saale), Germany. uli.schmidt@biochemtech.uni-halle.de
Journal of Virology
|January 22, 2000
Abstract:
VP1 is the major viral coat protein of murine polyomavirus and can be used for the generation of virus-like particles in vitro. Here, we demonstrate that capsid assembly is an equilibrium reaction followed by oxidation of intracapsomere disulfide bonds, which are not essential for the formation of virus-like particles but enable complete particle assembly and prevent capsid disassembly.