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Molecular cloning and mapping of a novel ADAM gene (ADAM29) to human chromosome 4

R Xu1, J Cai, T Xu

  • 1State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai, 200433, People's Republic of China.

Genomics
|January 25, 2000
PubMed

Insights

Researchers identified a novel human ADAM29 gene, highly expressed in the testis. This gene, encoding a protein with a disintegrin and metalloprotease domain, is located on chromosome 4.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The ADAM (a disintegrin and metalloprotease domain) family comprises type I integral membrane proteins.
  • ADAM proteins are crucial for biological processes like fertilization and myoblast fusion, involving cell-cell and cell-matrix interactions.

Purpose of the Study:

  • To report the cDNA sequence of a novel human ADAM gene, designated ADAM29.
  • To investigate the expression pattern and chromosomal localization of the ADAM29 gene.

Main Methods:

  • cDNA sequencing to determine the gene sequence.
  • Northern blot analysis to assess mRNA expression levels.
  • Radiation hybrid panel mapping for gene localization.

Main Results:

  • The cDNA sequence of the novel human ADAM29 gene was identified, featuring a putative fusion peptide.
  • Northern blot analysis demonstrated high expression of ADAM29 mRNA in human testis tissue.
  • Radiation hybrid panel mapping localized the ADAM29 gene to human chromosome 4q34.2-qter.

Conclusions:

  • A new member of the ADAM gene family, ADAM29, has been characterized.
  • ADAM29 exhibits tissue-specific expression, predominantly in the testis.
  • The gene's location on chromosome 4 provides a basis for further genetic studies.

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