Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequential MCM/P1 subcomplex assembly is required to form a heterohexamer with replication licensing activity.

T A Prokhorova1, J J Blow

  • 1Cancer Research Campaign Chromosome Replication Research Group, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom.

The Journal of Biological Chemistry
|January 25, 2000
PubMed
Summary

Replication licensing factor (RLF) ensures DNA replicates once per cell cycle. Researchers discovered a new assembly pathway for the MCM/P1 protein complex, crucial for DNA replication and chromatin binding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The living Barents Sea response to peak-warming and subsequent cooling.

Scientific reports·2025
Same author

[Proinflammatory activity of monocytes and activity of glutathione-dependent enzymes in red blood cells in women with depressive conditions].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2024
Same author

[Clinical and psychopathological characteristics of patients with late-onset schizophrenia and schizophrenia-like psychoses in clusters identified by biological parameters].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2024
Same author

[Activity of enzymes of glutamate, energy and glutathione metabolism in the first juvenile depression with attenuated symptoms of schizophrenia].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2022
Same author

[New approaches to the typology and prediction of the effectiveness of therapy for late-life depression].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2022
Same author

[Platelet glutathione reductase and glutathione-S-transferase in elderly patients with depression].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2021

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Replication licensing factor (RLF) controls DNA replication frequency.
  • RLF comprises RLF-M and RLF-B components.
  • RLF-M contains MCM/P1 proteins, but their complex composition and function were unclear.

Purpose of the Study:

  • To elucidate the composition and assembly of MCM/P1 protein complexes within RLF-M.
  • To characterize the interactions and subunit organization of MCM/P1 subcomplexes.
  • To determine the functional significance of the MCM/P1 heterohexamer assembly pathway for DNA replication.

Main Methods:

  • Analysis of Xenopus egg extracts.
  • Protein complex purification and characterization.
  • Investigation of protein-protein interactions and subunit composition.

Related Experiment Videos

Main Results:

  • MCM/P1 proteins primarily form heterohexamers in Xenopus extracts.
  • Detailed characterization of subcomplexes formed upon heterohexamer dissociation.
  • Identification of an ordered, multistep pathway for heterohexamer reformation from subcomplexes.

Conclusions:

  • The full MCM/P1 heterohexamer is essential for RLF-M activity.
  • Productive chromatin binding and DNA replication depend on the complete heterohexamer.
  • A novel assembly pathway is critical for ensuring once-per-cell-cycle DNA replication.