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Replication of Aleutian mink disease parvovirus in mink lymph node histocultures
K T Jensen1, J B Wolfinbarger, B Aasted
1Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT 59840, USA.
Abstract:
Aleutian mink disease parvovirus (ADV), causes an immune disorder with a persistent infection of lymphoid organs in adult mink. We studied replication of ADV in gel-supported histocultures prepared from adult mink mesenteric lymph node (MLN). Evidence of virus replication in the histocultures was first observed by indirect immunofluorescence 72 h after incubation with virus. Cells resembling lymphocytes and macrophages contained both ADV capsid (VP2) and nonstructural (NS1 and NS2) proteins, and were present in a distribution suggestive of infected cells within germinal centres. ADV replicative form and encapsidated virion DNA were also detected in infected histocultures at time-points after 72 h. In addition, we were able to passage ADV-Utah to a new round of histocultures. These results suggested that the infected cells were actual target cells for ADV replication and that productive ADV-Utah replication, complete with the generation of virus, was occurring in the histocultures. The mink MLN histocultures provide a system to study the replication and molecular pathogenesis of ADV in target tissues.
Insights
Aleutian mink disease parvovirus (ADV) replicates in mink lymph node histocultures, demonstrating productive viral replication in target cells. This model aids studying ADV
Area of Science:
- Veterinary Virology
- Immunopathology
- Infectious Disease Models
Background:
- Aleutian mink disease parvovirus (ADV) causes persistent immune disorders in mink.
- Lymphoid organs are primary targets for ADV infection.
Purpose of the Study:
- To investigate ADV replication in a controlled ex vivo system.
- To characterize ADV target cells and replication dynamics in mink mesenteric lymph nodes (MLN).
Main Methods:
- Utilized gel-supported histocultures of adult mink MLN.
- Employed indirect immunofluorescence to detect viral proteins (VP2, NS1, NS2).
- Analyzed viral DNA (replicative form and virion DNA) and performed virus passaging.
Main Results:
- ADV replication was confirmed by 72 hours post-incubation.
- Infected lymphocytes and macrophages were identified within germinal centers.
- Productive replication and virus generation were observed, with successful passaging to new histocultures.
Conclusions:
- Mink MLN histocultures effectively model ADV replication.
- This system supports the study of ADV molecular pathogenesis in target tissues.
- Identified lymphocytes and macrophages as key target cells for ADV.