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Related Experiment Videos

A multiple electrospray interface for parallel mass spectrometric analyses of compound libraries.

T Wang1, J Cohen, D B Kassel

  • 1CombiChem, Inc. 9050 Camino Santa Fe, San Diego, CA 92121, USA.

Combinatorial Chemistry & High Throughput Screening
|January 25, 2000
PubMed
Summary

A novel parallel spray interface for mass spectrometry allows sequential sampling from multiple High-Performance Liquid Chromatography columns. This advancement enhances throughput for combinatorial library analysis and profiling.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Chromatography

Background:

  • High-throughput analysis is crucial for combinatorial chemistry.
  • Existing parallel liquid chromatography-mass spectrometry (LC-MS) interfaces have limitations in sampling capacity or speed.

Purpose of the Study:

  • To develop and describe a new parallel spray interface for mass spectrometry.
  • To enable independent and sequential sampling from an array of High-Performance Liquid Chromatography (HPLC) columns.

Main Methods:

  • A parallel electrospray interface with an array of needles was designed.
  • A stepping motor and rotating plate assembly controlled sequential sampling from up to eight HPLC columns.
  • Quadrupole mass spectrometry was operated in single ion monitoring (SIM) mode for data acquisition.

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Main Results:

  • The interface successfully sampled effluent from multiple HPLC columns sequentially.
  • Good sensitivity and peak shape were maintained during data acquisition.
  • The system demonstrated the ability to sample each position multiple times per second.

Conclusions:

  • The developed parallel spray interface is effective for sequential sampling from multiple HPLC columns.
  • This technique shows promise for high-throughput combinatorial library analysis and profiling.
  • The interface offers improved sampling flexibility compared to simultaneous sampling systems.