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Related Experiment Videos

Gene expression during adventitious root formation in apple.

E Butler1, T F Gallagher

  • 1Botany Department, University College Dublin, Ireland.

Symposia of the Society for Experimental Biology
|January 25, 2000
PubMed
Summary
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Researchers identified key genes involved in adventitious root formation in apple trees using a novel model system. This study pinpoints specific transcripts, like 2-oxoacid dependent dioxygenase, that are crucial for initiating root development after auxin treatment.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Adventitious root formation is crucial for vegetative propagation in many woody plants.
  • Understanding the molecular mechanisms underlying this process is key to improving horticultural practices.
  • Apple (Malus domestica) cultivar Jork 9 provides a suitable model for studying root induction.

Purpose of the Study:

  • To identify transcripts differentially expressed during auxin-induced adventitious root formation in apple stem tissues.
  • To characterize novel genes involved in the early stages of root development.
  • To establish a reliable model system for studying adventitious rooting in woody plants.

Main Methods:

  • Utilized a model system with 1 mm apple stem disks treated with indole butyric acid (IBA).

Related Experiment Videos

  • Employed differential messenger RNA display (DDRT) and mRNA representational difference analysis (RDA) to identify differentially expressed genes.
  • Verified transcript expression using reverse northern blot and northern blot analyses.
  • Sequenced partial cDNAs to determine tentative gene identities.

    • Main Results:

    • Identified several clones exhibiting differential expression during auxin-induced root formation, including both up-regulated and down-regulated transcripts.
    • Confirmed expression patterns through rigorous blot analyses.
    • Discovered homology to polygalacturonase and MAP kinases among up-regulated transcripts.
    • Characterized a full-length cDNA for 2-oxoacid dependent dioxygenase, the most abundant up-regulated mRNA, expressed between 24-72 hours post-IBA treatment.

    Conclusions:

    • The study successfully identified key transcripts involved in adventitious root formation in apple.
    • The characterized 2-oxoacid dependent dioxygenase represents a significant finding in understanding early root development.
    • The established model system is effective for future molecular studies on adventitious rooting in woody plants.