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Related Experiment Videos

Mycobacteriophage TM4: genome structure and gene expression.

M E Ford1, C Stenstrom, R W Hendrix

  • 1Pittsburgh Bacteriophage Institute, USA.

Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
|January 25, 2000
PubMed
Summary

Mycobacteriophage TM4

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Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Mycobacteriophage TM4 is a double-stranded DNA (dsDNA) tailed phage.
  • It infects both fast-growing and slow-growing mycobacteria.
  • TM4 is utilized for genetic manipulation of mycobacteria, but its genetics are poorly understood.

Purpose of the Study:

  • To sequence and analyze the complete genome of mycobacteriophage TM4.
  • To understand the genetic organization and evolutionary relationships of TM4.
  • To elucidate the gene expression patterns following TM4 infection.

Main Methods:

  • Whole-genome sequencing of TM4.
  • Bioinformatic analysis of the TM4 genome.
  • Comparative genomics with other bacteriophages.

Main Results:

  • The complete 52,797 bp genome sequence of TM4 was determined.
  • TM4 shares common ancestry with other mycobacteriophages like L5 and D29.
  • TM4 encodes proteins similar to haloperoxidases, glutaredoxins, and WhiB transcriptional regulators.
  • TM4 gene expression follows a temporal pattern, with structural genes expressed late.

Conclusions:

  • The TM4 genome sequence provides a foundation for understanding its molecular biology.
  • TM4's genetic makeup reveals evolutionary connections to other phages.
  • Knowledge of TM4 genetics will enhance its utility as a tool for mycobacterial genetic engineering.

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