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Hypertonic saline resuscitation abrogates neutrophil priming by mesenteric lymph
G Zallen1, E E Moore, D Y Tamura
1Department of Surgery, Denver Health Medical Center, University of Colorado Health Science Center, USA.
The Journal of Trauma
|January 27, 2000
Summary
Hypertonic saline (HTS) resuscitation after hemorrhagic shock prevents neutrophil priming by mesenteric lymph. This finding suggests a mechanism for HTS
Area of Science:
- Trauma and critical care medicine
- Immunology
- Surgical research
Background:
- Neutrophil (PMN) priming post-hemorrhagic shock predicts multiple organ failure.
- Mesenteric lymph from shock animals resuscitated with lactated Ringer's (LR) primes PMNs and causes lung injury.
- Hypertonic saline (HTS) resuscitation protects against lung injury after hemorrhagic shock.
Purpose of the Study:
- To investigate if HTS resuscitation abolishes PMN priming by post-shock mesenteric lymph.
- To elucidate the mechanism behind HTS's protective effects in hemorrhagic shock.
Main Methods:
- Male rats underwent hemorrhagic shock and resuscitation with LR or HTS.
- Mesenteric lymph duct catheterization was performed.
- PMN priming for superoxide production was measured after fMLP activation.
Main Results:
- LR resuscitation yielded more mesenteric lymph than HTS.
- Mesenteric lymph from LR animals significantly primed PMNs.
- HTS resuscitation eliminated PMN priming by mesenteric lymph.
Conclusions:
- HTS decreases post-shock mesenteric lymph production.
- HTS eliminates PMN priming by mesenteric lymph.
- This suggests a mechanism for the beneficial effects of HTS resuscitation.