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cDNA sequence of bovine thioredoxin.
1Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 27, 2000
Summary
Researchers sequenced bovine thioredoxin complementary DNA (cDNA). Bovine thioredoxin shows high amino acid sequence conservation across mammals, highlighting its evolutionary importance.
Area of Science:
- Biochemistry
- Molecular Biology
- Genomics
Background:
- Thioredoxin is a crucial protein involved in redox homeostasis.
- The thioredoxin cDNA sequences of only five mammalian species were previously available in GenBank.
- Understanding mammalian thioredoxin diversity is important for comparative genomics.
Purpose of the Study:
- To determine and report the full-length cDNA sequence of bovine thioredoxin.
- To compare the bovine thioredoxin sequence with those of other mammalian species.
- To analyze the evolutionary conservation of thioredoxin at the amino acid level.
Main Methods:
- Reverse Transcription Polymerase Chain Reaction (RT-PCR)
- 5' and 3' Rapid Amplification of Complementary DNA Ends (RACE) methods
- Sequence comparison and multiple amino acid sequence alignment
Main Results:
- The full-length cDNA sequence of bovine thioredoxin was successfully determined.
- Sequence comparison revealed high conservation of thioredoxin amino acid sequences among mammalian species.
- Only one amino acid difference was found between bovine and ovine thioredoxin.
Conclusions:
- The cDNA sequence of bovine thioredoxin has been elucidated.
- Bovine thioredoxin is highly conserved among mammals, suggesting a conserved function.
- This finding contributes to the understanding of thioredoxin evolution in mammals.