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Related Experiment Videos

Nickel transport systems in microorganisms.

T Eitinger1, M A Mandrand-Berthelot

  • 1Humboldt-Universität zu Berlin, Institut für Biologie/Mikrobiologie, Germany. thomas.eitinger@rz.hu-berlin.de

Archives of Microbiology
|January 27, 2000
PubMed
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Isolation and characterization of the nikR gene encoding a nickel-responsive regulator in Escherichia coli.

Journal of bacteriology·1999

Nickel (Ni) is crucial for enzymes in all life. Bacteria utilize two main high-affinity nickel transporters, Nik system and HoxN permease, for uptake and to prevent toxicity.

Area of Science:

  • Biochemistry
  • Microbiology
  • Molecular Biology

Background:

  • Nickel (Ni) is an essential cofactor for numerous enzymatic reactions in prokaryotes and eukaryotes.
  • High-affinity Ni2+ transporters are vital for cellular Ni homeostasis.

Purpose of the Study:

  • To investigate the molecular mechanisms of bacterial nickel (Ni2+) transport systems.
  • To characterize the roles of NikA and HoxN in Ni2+ uptake and regulation.

Main Methods:

  • Molecular analyses of Ni2+ transporters.
  • Site-directed mutagenesis experiments.
  • Analysis of gene expression regulation by Ni-responsive repressors.

Main Results:

  • Identified two major bacterial Ni2+ transporter types: ABC transporter (Nik system) and a novel permease family (HoxN).

Related Experiment Videos

  • NikA exhibits dual roles in Ni2+ uptake and negative chemotaxis.
  • NikR repressor controls nik operon expression, similar to Fur.
  • HoxN family members identified across diverse bacteria and yeast, with varying specificities.
  • Essential transport residues identified via mutagenesis.

    • Conclusions:

    • Bacterial cells possess sophisticated Ni2+ uptake and export systems to manage Ni homeostasis.
    • Understanding these systems is crucial for microbial physiology and biotechnology.