Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Progress in understanding structure-function relationships in respiratory chain complex II.

B A Ackrell1

  • 1Molecular Biology Division, D.V.A. Medical Center and Department of Biochemistry and Biophysics, University of California, 4150 Clement Street, San Francisco, CA, USA. baca@itsa.ucsf.edu

FEBS Letters
|January 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enzyme electrokinetics: energetics of succinate oxidation by fumarate reductase and succinate dehydrogenase.

Biochemistry·2001
Same author

Late-onset optic atrophy, ataxia, and myopathy associated with a mutation of a complex II gene.

Annals of neurology·2000
Same author

Voltammetric studies of bidirectional catalytic electron transport in Escherichia coli succinate dehydrogenase: comparison with the enzyme from beef heart mitochondria.

Biochimica et biophysica acta·1999
Same author

Serum antibodies against the flavoprotein subunit of succinate dehydrogenase are sensitive markers of eye muscle autoimmunity in patients with Graves' hyperthyroidism.

The Journal of clinical endocrinology and metabolism·1999
Same author

Thyroid-associated ophthalmopathy in black South African patients with Graves' disease: relationship to antiflavoprotein antibodies.

Thyroid : official journal of the American Thyroid Association·1998
Same author

Eye muscle antibodies in patients with ocular myasthenia gravis: possible mechanism for eye muscle inflammation in acetylcholine-receptor antibody-negative patients.

Clinical immunology and immunopathology·1998

Complex II, a key enzyme linking the citric acid cycle and electron transport, is poorly understood. Recent X-ray structures of similar enzymes suggest protein movement during catalysis, offering new insights into flavoenzyme function.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Complex II (succinate:quinone oxidoreductase) is crucial for aerobic respiration, linking the citric acid cycle to the electron transport chain.
  • Its precise reaction mechanisms and interactions remain incompletely elucidated.
  • Complex II shares structural and catalytic similarities with anaerobic quinol:fumarate oxidoreductases.

Purpose of the Study:

  • To investigate the structure-function relationships of Complex II.
  • To gain insights into the catalytic mechanisms of flavoenzymes.
  • To explore potential protein movement during enzymatic catalysis.

Main Methods:

  • Comparative analysis of X-ray structures.
  • Structural and catalytic property assessment.

Related Experiment Videos

  • Flavoenzyme mechanism investigation.

    • Main Results:

    • X-ray structures of anaerobic quinol:fumarate oxidoreductases provide a model for Complex II.
    • Structural similarities suggest conserved functional mechanisms.
    • Evidence indicates potential protein movement during catalysis in this enzyme class.

    Conclusions:

    • X-ray crystallography of related enzymes offers valuable insights into Complex II function.
    • Protein dynamics may play a significant role in the catalytic cycle of flavoenzymes.
    • Further research into Complex II structure and dynamics is warranted.