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Molecular cloning and functional expression of Ca(v)3.1c, a T-type calcium channel from human brain
L L Cribbs1, J C Gomora, A N Daud
1Department of Physiology and the Cardiovascular Institute, Loyola University Medical Center, Maywood, IL, USA.
Abstract:
Low voltage-activated T-type calcium channels are encoded by a family of at least three genes, with additional diversity created by alternative splicing. This study describes the cloning of the human brain alpha1G, which is a novel isoform, Ca(v)3.1c. Comparison of this sequence to genomic sequences deposited in the GenBank allowed us to identify the intron/exon boundaries of the human CACNA1G gene. A full-length cDNA was constructed, then used to generate a stably-transfected mammalian cell line. The resulting currents were analyzed for their voltage- and time-dependent properties. These properties identify this gene as encoding a T-type Ca(2+) channel.
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