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Updated: Oct 17, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods
Published on: December 9, 2022
Effect of catalase on hydrogen peroxide penetration into Pseudomonas aeruginosa biofilms
1Center for Biofilm Engineering, Montana State University-Bozeman, Bozeman, Montana 59717-3980, USA. phil_s@erc.montana.edu
Abstract:
The penetration of hydrogen peroxide into biofilms formed by wild-type and catalase-deficient Pseudomonas aeruginosa strains was measured using microelectrodes. A flowing stream of hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked out. Catalase protects aggregated bacteria by preventing full penetration of hydrogen peroxide into the biofilm.
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