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Observation of soft contact lens disinfection with fluorescent metabolic stains
J Gavin1, N F Button, I A Watson-Craik
1School of Biological and Biomedical Sciences, United Kingdom.
Applied and Environmental Microbiology
|February 2, 2000
Abstract:
A rapid fluorescent staining method using a tetrazolium dye and propidium iodide for the in-use assessment of disinfection of Pseudomonas aeruginosa biofilms on soft contact lenses showed that 11 to 13% of cells on lenses remained actively respiring and recoverable by culture methods after 30 min of exposure to 3% hydrogen peroxide.