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Related Experiment Videos

Unbinding-binding transition induced by molecular snaps in model membranes.

N Taulier1, C Nicot, M Waks

  • 1Laboratoire d'Imagerie Paramétrique, UMR 7623 CNRS, Université Pierre et Marie Curie, 15 rue de l'Ecole de Médecine, 75270 Paris cédex 06, France.

Biophysical Journal
|February 2, 2000
PubMed
Summary

This study reveals how macromolecular inclusions, like proteins and peptides, alter model membrane spacing. These "macromolecular snaps" change interlamellar distance based on their surface concentration and length, not just membrane volume.

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Area of Science:

  • Supramolecular Chemistry
  • Materials Science
  • Biophysics

Background:

  • Model membranes, specifically lamellar phases of nonionic surfactants, dodecane, and water, are crucial for studying membrane interactions.
  • The interlamellar distance in these systems typically depends on the membrane volume fraction (Phi).

Purpose of the Study:

  • To investigate the effect of macromolecular inclusions acting as membrane binders (macromolecular snaps) on lamellar phase structure.
  • To determine how the length and concentration of these inclusions influence interlamellar spacing and membrane interactions.

Main Methods:

  • Utilizing a lamellar phase model system composed of a nonionic surfactant, dodecane, and water.
  • Introducing transmembrane proteins and de novo designed peptides of varying lengths and compositions as macromolecular inclusions.

Related Experiment Videos

  • Analyzing changes in interlamellar distance and fitting experimental data to a thermally stabilized membrane model.

    • Main Results:

    • Macromolecular inclusions induce a binding transition in the lamellar phase, altering interlamellar distance.
    • The interlamellar distance becomes dependent on the surface concentration of snaps, not solely on the membrane volume fraction (Phi).
    • Inclusions with hydrophobic segments require a length at least equal to the membrane's hydrophobic length to be effective; model parameters must account for inclusion length.

    Conclusions:

    • Macromolecular inclusions significantly modify the structural organization of lamellar phases by acting as membrane-bridging elements.
    • The effectiveness of these inclusions is dictated by their specific characteristics, including length and hydrophobic interactions.
    • A refined model incorporating inclusion length is necessary for accurately predicting membrane interactions in the presence of such components.