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[The promoter sequence of human nitric-oxide-synthase gene: functional study]
1The Medical School of Jamusi University, Jamusi, Heilongjiang, 154003 P.R. China. XianFengZ@hotmail.com
Objective:
Endothelial nitric oxide synthase (ecNOS) catalyzes the NADPH and O(2) to nitric oxide(NO). This study aimed at the regulation mechanism of the transcription of ecNOS gene.
Methods:
The promoter regions of ecNOS gene were analyzed by gel mobility shift assay and DNase I footprinting assay using nuclear extract from endothelial cells.
Results:
Three regions were protected by protein/transcription-factors. One of these regions, (-106 to -88) was demonstrated to be SP1 binding consensus sequence which was extremely rich in GC content. The other two regions, (-79 to -64) and (-58 to -44), covered the CTCF binding regions, but showed various binding modes.
Conclusion:
The protected regions are bound either with aggregated SP1/SP1- like proteins or with new transcription factor which have not been reported yet.