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[Induction of apoptosis in ovarian carcinoma cells by HSP70 antisense oligodeoxynucleotides]
1Department of Obstetrics and Gynecology, Second Affliated Hospital, West China University of Medical Sciences, Chengdu, Sichuan, 610041 P.R. China. yuqua wei@mail.sc.cninfo.net
Objective:
To investigate the role of HSP70 in the proliferation and survival of ovarian carcinoma cells by inhibiting HSP70 expression with HSP70 antisense oligomer.
Methods:
Morphological changes of apoptotic cells were investigated by light microscopy. DNA fragmentation was analysed by agarose gel electrophoresis. Kinetics of induction of apoptosis and cell cycle were analysed by flow cytometry.
Results:
The HSP70 antisense oligomer treated ovarian carcinoma cells showed apparent inhibition of proliferation and characteristic morphological changes of apoptosis. Also, a ladder-like pattern of DNA fragments was demonstrated on agarose gel electrophoresis. HSP70 antisense-oligomer induced apoptosis of ovarian carcinoma cells mainly in G(1)/S phase in 8.3% to 41% at 1 to 20micromol/L. The apoptosis-inducting effect of HSP70 antisense oligomer was in a dose- and time-dependent manner.
Conclusion:
HSP70 antisense oligomer could not only inhibit the proliferation but also induce the apoptosis in ovarian carcinoma cells.
Insights
HSP70 antisense oligomer effectively inhibits ovarian carcinoma cell proliferation and induces apoptosis. This targeted approach offers a promising strategy for ovarian cancer treatment.
Area of Science:
- Molecular Biology
- Cell Biology
- Oncology
Context:
- Ovarian carcinoma is a leading cause of cancer-related deaths in women.
- Heat shock protein 70 (HSP70) plays a critical role in cancer cell survival and proliferation.
- Targeting HSP70 presents a potential therapeutic strategy for ovarian cancer.
Purpose:
- To investigate the role of HSP70 in ovarian carcinoma cell proliferation and survival.
- To evaluate the efficacy of HSP70 antisense oligomer in inhibiting HSP70 expression.
- To assess the impact of HSP70 inhibition on apoptosis and cell cycle progression.
Summary:
- Treatment with HSP70 antisense oligomer resulted in significant inhibition of ovarian carcinoma cell proliferation.
- Morphological analysis and DNA fragmentation assays confirmed the induction of apoptosis.
- Flow cytometry revealed that HSP70 antisense oligomer induced apoptosis in the G(1)/S phase in a dose- and time-dependent manner.
Impact:
- HSP70 antisense oligomer demonstrates potential as a therapeutic agent for ovarian cancer.
- Inhibition of HSP70 may represent a novel strategy to overcome treatment resistance in ovarian carcinoma.
- Further research into HSP70-targeted therapies could lead to improved patient outcomes.