Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tumour phospholipid metabolism.

F Podo1

  • 1Laboratory of Cell Biology, Istituto Superiore di Sanità, Rome, Italy.fpodo@iss.it

NMR in Biomedicine
|February 2, 2000
PubMed
Summary

Magnetic resonance spectroscopy (MRS) reveals phospholipid metabolites like phosphocholine (PCho) and phosphoethanolamine (PEtn) as key indicators of tumor progression and treatment response. These findings aid in understanding cancer biochemistry and developing targeted therapies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monitoring response to cytostatic cisplatin in a HER2(+) ovary cancer model by MRI and in vitro and in vivo MR spectroscopy.

British journal of cancer·2013
Same author

Choline kinase-alpha by regulating cell aggressiveness and drug sensitivity is a potential druggable target for ovarian cancer.

British journal of cancer·2013
Same author

Temperature dependence of intracellular pH in higher plant cells : A (31)P nuclear magnetic resonance study on maize root tips.

Planta·2013
Same author

The interaction with phospholipids of bee venom melittin: a structural study of the Peptide and lipid components.

Biophysical journal·2009
Same author

Indications for breast magnetic resonance imaging. Consensus document "Attualità in senologia", Florence 2007.

La Radiologia medica·2008
Same author

MR spectroscopy of the breast.

La Radiologia medica·2008

Area of Science:

  • Biochemistry
  • Oncology
  • Medical Imaging

Background:

  • Phospholipid (PL) precursors and catabolites, detected via magnetic resonance spectroscopy (MRS), have shown potential as biochemical indicators of tumor progression and therapy response since the 1980s.
  • Recent MRS analyses of 31P phosphomonoester (PME) and phosphodiester (PDE) resonances, along with 1H 'choline peak', have validated these expectations.
  • Absolute quantification of these signals allows for deeper investigation into the biochemical mechanisms governing intracellular PL derivative pools in tumors.

Purpose of the Study:

  • To provide an overview of quantitative MRS measurements of phosphocholine (PCho), phosphoethanolamine (PEtn), and their glycerol derivatives (GPC, GPE) in human tumors and cells.
  • To explore correlations between MRS parameters (e.g., PEtn/PCho, PCho/GPC ratios) and in vitro cell growth status or tumorigenicity.
  • To review current and novel hypotheses regarding the role of choline and ethanolamine metabolic pathways in modulating intracellular PCho and PEtn pools in response to stimuli or malignant transformation.

Main Methods:

  • Quantitative MRS measurements of specific phospholipid metabolites (PCho, PEtn, GPC, GPE).
  • Analysis of human tumors (breast, brain, lymphomas) and cells, alongside normal mammalian tissues.
  • Correlation analysis of MRS-derived ratios (PEtn/PCho, PCho/GPC) with cell growth and tumorigenicity data.

Main Results:

  • Quantitative MRS provides insights into the levels of PCho, PEtn, GPC, and GPE in various human cancers and normal tissues.
  • Specific MRS ratios (e.g., PEtn/PCho) show potential correlations with tumor cell proliferation and malignancy.
  • The study highlights the significance of these phospholipid metabolites as biomarkers in oncology.

Conclusions:

  • Quantitative MRS of phospholipid metabolites offers valuable insights into tumor biochemistry and progression.
  • Metabolite ratios derived from MRS can serve as potential indicators of tumor cell growth and malignancy.
  • Understanding the interplay of choline and ethanolamine pathways is crucial for interpreting MRS findings in cancer.

Related Experiment Videos