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Endomysial antibody production is not related to histological damage after in vitro gluten challenge
F Biagi1, N D Parnell, H J Ellis
1Gastroenterology Unit, GKT, St Thomas' Hospital, London, UK.
Objective:
To determine whether in vitro induction of endomysial antibodies is an in vitro marker of coeliac toxicity.
Design:
To determine whether in vitro endomysial antibodies induced by gliadin incubation correlate with histological damage induced by in vitro gliadin challenge.
Methods:
Small-bowel organ cultures from seven patients with treated coeliac disease were incubated in an organ culture system with gliadin; histological damage was morphometrically evaluated and endomysial antibodies were measured in the organ culture supernatant by indirect immunofluorescence.
Results:
Although incubation with gliadin caused histological damage, there was no detectable production of endomysial antibody.
Conclusions:
In vitro, endomysial antibody induction cannot be used as a marker of coeliac toxicity. Endomysial antibodies are not necessary for generating the histological lesion of coeliac disease.
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