Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Detergent Purification of Membrane Proteins

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Endotoxin removal by affinity sorbents.

Journal of biochemical and biophysical methods·2001

Same author

Preparative two-step purification of recombinant human basic fibroblast growth factor from high-cell-density cultivation of Escherichia coli.

Journal of chromatography. B, Biomedical sciences and applications·2000

Same author

Expanded-bed chromatography in primary protein purification.

Journal of chromatography. A·2000

Same author

Continuous-bed chromatography for the analysis and purification of recombinant human basic fibroblast growth factor.

Journal of chromatography. A·1999

Same author

Selective adsorption of endotoxin inside a polycationic network of flat-sheet microfiltration membranes.

Journal of molecular recognition : JMR·1999

Same author

Endotoxin removal with poly(ethyleneimine)-immobilized adsorbers: Sepharose 4B versus flat sheet and hollow fibre membranes.

Journal of chromatography. B, Biomedical sciences and applications·1998

Same journal

High-level De novo Biosynthesis of Plant-Derived Pinosylvin in Yarrowia lipolytica via Metabolic Engineering and Surfactant-Mediated Fermentation.

Journal of biotechnology·2026

Same journal

The effect of reduced graphene oxide in chitosan-based nanoparticles on the enzymatic properties of the immobilized enzyme.

Journal of biotechnology·2026

Same journal

High-level biosynthesis of gastrodin in engineered Escherichia coli.

Journal of biotechnology·2026

Same journal

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing.

Journal of biotechnology·2026

Same journal

Development of an inducible cellobiohydrolase promoter and its application for enhancing the production of ganoderic acids in Ganoderma lingzhi.

Journal of biotechnology·2026

Same journal

Malic acid and allied exogenous chemicals induces desaturase gene expression and elevates PUFA production in marine microalgae Isochrysis sp.

Journal of biotechnology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 1, 2026

Overexpressing and Purifying a Toxic Nuclease from Escherichia coli

Overexpressing and Purifying a Toxic Nuclease from Escherichia coli

Published on: August 29, 2025

Endotoxin removal from protein solutions.

D Petsch¹, F B Anspach

¹Biochemical Engineering Division, GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.

Journal of Biotechnology

|February 3, 2000

Summary

This summary is machine-generated.

Endotoxin contamination in protein solutions is a significant challenge. This review explores various methods for endotoxin removal, crucial for safe parenteral drug administration.

More Related Videos

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Published on: September 29, 2019

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Published on: February 16, 2010

Related Experiment Videos

Last Updated: Mar 1, 2026

Overexpressing and Purifying a Toxic Nuclease from Escherichia coli

Overexpressing and Purifying a Toxic Nuclease from Escherichia coli

Published on: August 29, 2025

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Published on: September 29, 2019

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies

Published on: February 16, 2010

Area of Science:

Biotechnology
Pharmaceutical Science
Microbiology

Background:

Gram-negative bacteria frequently contaminate protein solutions with toxic endotoxins.
Endotoxin removal is critical for safe parenteral administration of biopharmaceutical products.
Existing water decontamination methods like ultrafiltration are ineffective for protein solutions.

Purpose of the Study:

To provide a comprehensive overview of endotoxin removal methods for protein solutions.
To discuss the properties of endotoxins and the significance of contamination.
To identify optimization strategies for endotoxin removal based on endotoxin characteristics.

Main Methods:

Review of existing literature and patent data on endotoxin removal techniques.
Discussion of ion-exchange chromatography, two-phase extraction, and affinity techniques.
Exploration of tailor-made endotoxin-selective adsorber matrices.

Main Results:

No universal method exists for endotoxin removal from protein solutions.
Current techniques, including ultrafiltration and patented methods, have limitations in broad applicability.
Affinity techniques and selective adsorbers show promise but require optimization.

Conclusions:

Effective endotoxin removal necessitates methods tailored to specific protein solutions and endotoxin properties.
Further research into optimizing selective adsorption and affinity-based strategies is warranted.
Developing broadly applicable and efficient endotoxin removal techniques remains a key challenge in biopharmaceutical manufacturing.