Related Experiment Videos
Tritiated kappa receptor antagonist norbinal torphimine: synthesis and in vitro binding in three different tissues
1Institute of Biochemistry and Isotope Laboratory, Biological Research Center, Hungarian Academy of Sciences, Szeged.
Life Sciences
|February 5, 2000
Abstract:
Recently a new antagonist with high selectivity for the kappa receptors (norbinaltorphimine) was developed and tested in various systems. This compound was radiolabelled with tritium resulting in high specific radioactivity (47.2 Ci/mmol). [3H]Norbinaltorphimine was characterized by in vitro radioligand binding assays. The radioligand binds to kappa-opioid receptors with a high potency and selectivity in guinea pig, frog and rat brain membranes. Our results suggest the kappa1 specificity of the radioligand.