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Related Experiment Video

Updated: Jul 5, 2026

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

Genes dependent on zebrafish cyclops function identified by AFLP differential gene expression screen.

A L Rubinstein1, D Lee, R Luo

  • 1Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA.

Genesis (New York, N.Y. : 2000)
|March 21, 2000
PubMed
Summary
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Researchers identified two novel genes, crestin and calreticulin, in zebrafish using a differential gene expression screen. Calreticulin

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • The zebrafish cyclops (cyc) gene encodes a Transforming Growth Factor beta (TGFbeta) signaling factor.
  • This factor is closely related to the mouse Nodal signaling pathway, crucial for embryonic development.
  • Understanding genes regulated by Cyc signaling is key to deciphering developmental processes.

Purpose of the Study:

  • To identify novel genes regulated by zebrafish Cyclops (cyc) signaling.
  • To characterize the expression patterns and regulation of newly identified genes in zebrafish embryos.
  • To explore the utility of differential gene expression screening in identifying signaling pathway targets.

Main Methods:

  • Differential gene expression screening using amplified fragment length polymorphisms (AFLP) comparing cyc mutant and wild-type zebrafish cDNA pools.

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Last Updated: Jul 5, 2026

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
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Published on: October 19, 2013

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  • Isolation and characterization of two novel genes: crestin and zebrafish calreticulin.
  • Analysis of gene expression patterns during zebrafish embryonic development, particularly in relation to cyc and one-eyed pinhead (oep) mutations.

    • Main Results:

    • Two previously undescribed zebrafish genes, crestin and calreticulin, were identified.
    • Crestin is expressed in neural crest cells and shows altered expression in cyc mutants.
    • Zebrafish calreticulin is highly expressed in tissues affected by cyc mutations (hatching gland, floor plate) and its expression during gastrulation is dependent on Cyc and Nodal signaling (oep).

    Conclusions:

    • Differential screening of cDNA from mutant and wild-type embryos is an effective method for discovering genes regulated by specific signaling pathways.
    • Zebrafish calreticulin is a novel target of Cyclops/Nodal signaling during gastrulation and development.
    • The findings provide new insights into the genetic network controlled by TGFbeta signaling in zebrafish development.