Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell proliferation and cell death in the developing chick inner ear: spatial and temporal patterns.

H Lang1, M M Bever, D M Fekete

  • 1Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.

The Journal of Comparative Neurology
|February 8, 2000
PubMed
Summary

This study maps cell division and death in developing chicken inner ears. It reveals complex regional differences and shows that outward growth isn't always linked to cell proliferation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adeno-associated virus-mediated gene delivery into the scala media of the normal and deafened adult mouse ear.

Gene therapy·2011
Same author

[The role of diffusion 3-Tesla MRI in detecting prostate cancer before needle biopsy: multiparametric study of 111 patients].

Journal de radiologie·2010
Same author

[Recommendations Onco-Urology 2010: Kidney cancer].

Progres en urologie : journal de l'Association francaise d'urologie et de la Societe francaise d'urologie·2010
Same author

LIM-class homeobox gene Lim1, a novel oncogene in human renal cell carcinoma.

Oncogene·2010
Same author

[Resectable colorectal liver metastases: pro neoadjuvant therapy - contra neoadjuvant therapy].

Zeitschrift fur Gastroenterologie·2010
Same author

Online signal processing of internal anal sphincter activity during pelvic autonomic nerve stimulation: a new method to improve the reliability of intra-operative neuromonitoring signals.

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2010

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Otolaryngology

Background:

  • Inner ear morphogenesis relies on a balance between cell proliferation and apoptosis.
  • Previous studies lacked systematic comparisons of these processes within individual developing ears.

Purpose of the Study:

  • To systematically compare spatial and temporal patterns of cell proliferation and apoptosis in the developing chicken otocyst.
  • To investigate the relationship between cell division, cell death, and regional growth during inner ear development.

Main Methods:

  • Utilized bromodeoxyuridine (BrdU) labeling to identify proliferating cells.
  • Employed nuclear condensation/fragmentation and terminal dUTP nick-end labeling (TUNEL) assays for apoptotic cells.
  • Mapped cell proliferation and death spatially and temporally across otocyst sections from stages 13-29.

Related Experiment Videos

Main Results:

  • Identified complex regional differences in otocyst epithelial proliferation.
  • Found that most "hot spots" of cell death spatially corresponded to regions of reduced proliferation.
  • Observed that the superior otocyst expands without high proliferation, decoupling growth from cell division.

Conclusions:

  • Inner ear morphogenesis involves intricate regional regulation of cell proliferation and apoptosis.
  • Outward expansion of the otic vesicle is not strictly dependent on concurrent cell proliferation in all regions.
  • This study provides a foundation for further research into the mechanisms controlling cell division and death in inner ear development.