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Related Experiment Videos

Normal limb development in conditional mutants of Fgf4.

A M Moon1, A M Boulet, M R Capecchi

  • 1Howard Hughes Medical Institute, Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.

Development (Cambridge, England)
|February 9, 2000
PubMed
Summary
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Fibroblast growth factor 4 (FGF4) is not essential for normal limb development in mice, contrary to previous hypotheses. Conditional knockout studies revealed that FGF4

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Fibroblast growth factors (FGFs) are crucial for vertebrate development.
  • FGF4 is expressed in the apical ectodermal ridge (AER) and hypothesized to regulate limb development.
  • Germline mutations in Fgf4 cause embryonic lethality, hindering study of its role in limb patterning.

Purpose of the Study:

  • To investigate the role of FGF4 in murine limb development using a conditional knockout approach.
  • To bypass embryonic lethality and assess FGF4 function specifically in the AER during limb formation.

Main Methods:

  • Generation of a conditional Fgf4 mutant using Cre/loxP technology for site-specific gene excision.
  • Inactivation of Fgf4 in AER-expressing cells, coupled with reporter gene activation.

Related Experiment Videos

  • Analysis of limb development, outgrowth, and patterning in conditional Fgf4 mutants.

    • Main Results:

    • Conditional Fgf4 mutants exhibited normal limb development, outgrowth, and patterning.
    • Expression of key developmental genes (Shh, Bmp2, Fgf8, Fgf10) remained unaffected in mutant limb buds.
    • The proposed FGF4-SHH feedback loop is not essential for coordinating limb development.

    Conclusions:

    • FGF4 is not essential for normal limb development in mice.
    • Other AER factors may compensate for FGF4 function in vivo.
    • The established FGF4-SHH feedback loop's role in limb patterning requires re-evaluation.