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Truncated TSG101 transcripts in human leukemia and lymphoma cell lines
1Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, 1-1 Kanakoden, Chikusa-ku, Nagoya 464-8681, Japan. yhosokaw@aichi-cc.pref.aichi.jp
Journal of Cancer Research and Clinical Oncology
|February 9, 2000
Summary
Truncated TSG101 transcripts are detectable in leukemia and lymphoma cell lines, but their low expression levels suggest they are not a major factor in these hematopoietic malignancies.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The TSG101 gene is implicated in cellular transformation.
- Aberrant TSG101 transcripts are found in solid tumors and hematopoietic malignancies.
Purpose of the Study:
- To investigate the presence and potential role of aberrant TSG101 transcripts in human leukemia and lymphoma cell lines.
Main Methods:
- Nested reverse transcription/polymerase chain reaction (RT-PCR) was used to detect TSG101 transcripts.
- Northern blot analysis confirmed expression levels.
- Sequencing identified transcript variations.
- Southern blot analysis assessed gene rearrangement.
Main Results:
- Wild-type TSG101 transcripts were abundant.
- Smaller, truncated TSG101 transcripts were detected in most leukemia and lymphoma cell lines, but at very low levels.
- Transcript variations showed extensive deletions but no clear association with specific hematopoietic disorders.
- No gross TSG101 gene rearrangements were found.
- Truncated transcripts were also present in normal leukocytes.
Conclusions:
- Truncated TSG101 transcripts are detectable in human leukemia and lymphoma cell lines.
- The low expression levels and presence in normal cells suggest these variant transcripts may not play a major role in the pathogenesis of these cancers.