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Updated: Aug 9, 2026

Protein Crystallization for X-ray Crystallography
Published on: January 17, 2011
Crystallization and preliminary X-ray analysis of Thermotoga maritima ribosome recycling factor
M Selmer1, S Al-Karadaghi, G Hirokawa
1Department of Molecular Biophysics, Center for Chemistry and Chemical Engineering, Lund University, Box 124, 22100 Lund, Sweden.
Abstract:
Thermotoga maritima ribosome recycling factor (RRF) is one of the proteins catalyzing the fourth step in prokaryotic protein synthesis, ribosome recycling. The RRF protein was crystallized with ammonium sulfate. Native diffraction data to 2.55 A resolution were obtained at the MAX II synchrotron from a flash-frozen crystal at 100 K. The crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 47, c = 298 A, and probably contain one monomer per asymmetric unit.
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