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Crystallization and preliminary X-ray diffraction analysis of Thermus thermophilus prolyl-tRNA synthetase
A Yaremchuk1, S Cusack, M Tukalo
1EMBL Grenoble Outstation, 156X, 38042 Grenoble CEDEX 9, France. yarem@embl-grenoble.fr
Acta Crystallographica. Section D, Biological Crystallography
|February 10, 2000
Abstract:
Prolyl-tRNA synthetase from Thermus thermophilus (ProRSTT) was purified to homogeneity using a five-step purification procedure and was crystallized using ethylene glycol as a precipitant. Crystals of ProRSTT belong to the space group P2(1)2(1)2, with unit-cell parameters a = 132, b = 191, c = 125 A, have two homodimers per asymmetric unit and diffract to 2.4 A resolution. A complete native data set to 2.43 A resolution has been collected and a data set from ProRSTT in complex with proline has been collected to 2.9 A resolution.