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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Crystallization and preliminary X-ray diffraction studies of a beta-carbonic anhydrase from the red alga Porphyridium
S Mitsuhashi1, T Mizushima, E Yamashita
1Marine Biotechnology Institute, Kamaishi Laboratories, 3-75-1, Heita, Kamaishi, Iwate 026-0001, Japan. mitsuhashi@kamaishi.mbio.co.jp
Acta Crystallographica. Section D, Biological Crystallography
|February 10, 2000
Abstract:
The beta-carbonic anhydrase from the red alga Porphyridium purpureum was heterologously expressed, purified and crystallized. The crystals belong to space group P2(1) (unit-cell parameters a = 63.8, b = 113.9, c = 73.8 A, beta = 104.1 degrees) with two subunits per asymmetric unit and diffract to 2.5 A resolution.
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