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Crystallization and preliminary crystallographic studies of ribosome recycling factor from Escherichia coli
1Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151--742, South Korea.
Acta Crystallographica. Section D, Biological Crystallography
|February 10, 2000
Abstract:
Ribosome recycling factor (RRF) catalyzes the disassembly of a termination complex during the final stage of protein synthesis. RRF from Escherichia coli has been crystallized with PEG 400 as precipitant at 287 K. The crystal belongs to the trigonal space group P3(1)21 (or P3(2)21), with unit-cell parameters a = b = 48.08, c = 141.67 A. Native data were collected from a frozen crystal to a resolution of 3.0 A on a Cu Kalpha rotating-anode X-ray source.