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Crystallization and preliminary X-ray analysis of a bacteriophage T4 primase fragment
I P Korndörfer1, J Salerno, D Jing
1Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, 1229 University of Oregon, Eugene, Oregon 97403-1229, USA.
Acta Crystallographica. Section D, Biological Crystallography
|February 10, 2000
Abstract:
The primase from bacteriophage T4 is a single-stranded DNA-dependent RNA polymerase that is one of the seven proteins that constitute the DNA-replication machinery of bacteriophage T4. In an attempt to crystallize the protein, a number of variants were generated. One such construct, which includes the C-terminal region (residues 196-340), gave four different crystal forms which diffract in the 3. 5-6.0 A resolution range.