Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression relevant to Down syndrome: problems and approaches.

F Tassone1, R Lucas, D Slavov

  • 1Eleanor Roosevelt Institute, Denver, CO, USA.

Journal of Neural Transmission. Supplementum
|February 10, 2000
PubMed
Summary

Researchers analyzed gene expression on chromosome 21 to identify genes linked to Down Syndrome. This expression analysis helps prioritize genes for further study, potentially reducing costs and time for Down Syndrome research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rates of Sexual Harassment Among Emergency Medicine Faculty and the Impact on Faculty Engagement.

Academic emergency medicine : official journal of the Society for Academic Emergency Medicine·2026
Same author

Diabetic retinopathy screening telemedicine-based compared with standard of care : an economic analysis in a pilot experience of Italian healthcare.

Acta diabetologica·2025
Same author

Endothelial ENaC as a repressor of oxidative stress and a guardian of lung capillary barrier function in bacterial and viral pneumonia.

Frontiers in physiology·2025
Same author

Real-life effectiveness and safety of baricitinib in patients with severe alopecia areata: A 24-week Italian study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

An observational cross-sectional study of pharmaceutical waste disposal practices in Australian medical imaging departments: A comparison of community versus hospital practice.

Radiography (London, England : 1995)·2024
Same author

The influence of neonatal BCG vaccination on in vitro cytokine responses to Plasmodium falciparum.

BMC immunology·2024

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • The long arm of human chromosome 21 (21q) contains numerous genes potentially contributing to the Down Syndrome phenotype.
  • Identifying specific 21q genes responsible for Down Syndrome requires targeted functional and phenotypic analysis, which is resource-intensive.

Purpose of the Study:

  • To prioritize 21q genes for further Down Syndrome research by analyzing their expression patterns.
  • To investigate gene expression levels and localization in normal versus trisomic cells and tissues.

Main Methods:

  • Expression analysis of forty-two 21q genes using Northern analysis, cDNA array screening, and RNA tissue in situ hybridization.
  • Comparison of gene expression in normal and trisomic cell lines and mouse tissues.

Related Experiment Videos

  • Analysis of alternative RNA processing for potential generation of brain-specific proteins.

    • Main Results:

    • Expression data (time, place, and level) were obtained for a subset of 21q genes.
    • Overexpression of certain genes in trisomic contexts was observed.
    • Alternative processing mechanisms were identified, suggesting the potential for brain-specific protein variants.

    Conclusions:

    • Expression profiling provides valuable information for prioritizing 21q genes relevant to Down Syndrome.
    • Several candidate genes warrant further investigation for their role in the Down Syndrome phenotype.
    • Interpreting gene expression data requires careful consideration of complexities and potential ambiguities.