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Interactions between neomycin and cerebral dopaminergic and serotoninergic transmission in rats

A el Ayadi1, M Errami

  • 1Department of Biology, Abdelmalek Essaâdi University, Morocco.

Therapie
|February 10, 2000
PubMed

Insights

Neomycin significantly alters dopamine and serotonin levels in rat brains, impacting neurotransmitter balance. This study reveals neomycin

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biochemistry

Background:

  • Monoamine neurotransmitters like dopamine and serotonin are crucial for brain function.
  • Neomycin is an antibiotic with potential neurological side effects that require investigation.

Purpose of the Study:

  • To investigate the impact of neomycin administration on monoamine levels in rat striatal tissue.
  • To elucidate the effects of neomycin on dopamine and serotonin pathways in the brain.

Main Methods:

  • Rats were administered neomycin (2 mg/kg; i.p.) and observed over 24 hours.
  • Striatal tissue homogenates were analyzed using High-Performance Liquid Chromatography (HPLC) with electrochemical detection.
  • Levels of dopamine, serotonin, and their metabolites were quantified.

Main Results:

  • Neomycin caused a significant increase in striatal dopamine levels (+35%) and a decrease in its metabolite levels (-36%).
  • Striatal serotonin levels increased significantly (+130%) within 2 hours post-injection.
  • Ratios of DOPAC/DA and 5HIAA/5HT were reduced, indicating altered neurotransmitter metabolism.

Conclusions:

  • Neomycin exerts an inhibitory effect on both dopaminergic and serotoninergic systems in the rat striatum.
  • Potential mechanisms include altered biosynthesis via enzyme stimulation (tyrosine hydroxylase, tryptophan hydroxylase) or modified enzyme activity (MAO, COMT).
  • Calcium channel blockade is also suggested as a possible mechanism for neomycin's neurochemical effects.

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