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Bacterial components inhibit fibroblast proliferation in vitro
E M Edds1, T M Bergamini, K R Brittian
1Department of Surgery, University of Louisville School of Medicine and Veterans Administration Medical Center, KY 40202, USA.
ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|February 10, 2000
Summary
Perigraft fluid from Staphylococcus epidermidis infected grafts inhibits fibroblast proliferation. This bacterial inhibitor is not dependent on host factors or graft material, suggesting a direct bacterial mechanism.
Area of Science:
- Microbiology
- Biomaterials Science
- Cell Biology
Background:
- Vascular graft infections can lead to complications.
- Bacterial factors may influence host tissue response around grafts.
Purpose of the Study:
- To investigate the effect of perigraft fluid from Staphylococcus epidermidis infected grafts on fibroblast proliferation.
- To identify the source and nature of fibroblast inhibitors in infected perigraft fluid.
Main Methods:
- Mouse model of infected vascular grafts.
- In vitro proliferation assay using murine fibroblasts (ATCC CCL-12) and tritiated thymidine.
- Testing of whole bacteria, disrupted bacteria, bacterial supernatants, and cell wall products from S. epidermidis and Staphylococcus aureus.
Main Results:
- Perigraft fluid from S. epidermidis infected grafts significantly inhibited fibroblast proliferation.
- Fibroblast inhibition was primarily dependent on bacteria, not host mediators or biomaterial.
- Disrupted bacteria and bacterial supernatants from S. epidermidis and S. aureus inhibited fibroblast proliferation.
- The inhibitor from disrupted slime-producing S. epidermidis was trypsin-heat resistant, while others were sensitive.
Conclusions:
- Components from both slime-producing and non-slime-producing S. epidermidis, as well as S. aureus, inhibit fibroblast proliferation.
- Bacterial factors, rather than host or graft material, are responsible for fibroblast inhibition.
- These findings suggest a direct bacterial mechanism impacting tissue integration around infected grafts.