Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

The immune response to apoptotic cells.

¹Laboratory for Cellular and Molecular Immunology, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Israel. mevdm@netvision.net.il

Annals of the New York Academy of Sciences

|February 11, 2000

Summary

Excessive or improperly processed apoptotic cells can trigger autoimmune responses, leading to conditions like lupus. This study shows how cell death remnants can generate autoantibodies in mice.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The embryotoxicity of sera from patients with autoimmune diseases on post-implantation rat embryos in culture persists during remission and is not related to oxidative stress.

Lupus·2010

Same author

Early diagnosis and treatment of atrioventricular block in the fetus exposed to maternal anti-SSA/Ro-SSB/La antibodies: a prospective, observational, fetal kinetocardiogram-based study.

Circulation·2009

Same author

Sacral herpes-zoster infection presenting as sciatic pain.

Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases·2008

Same author

'Danger' effect of low-density lipoprotein (LDL) and oxidized LDL on human immature dendritic cells.

Clinical and experimental immunology·2007

Same author

Reduced placental growth and hCG secretion in vitro induced by antiphospholipid antibodies but not by anti-Ro or anti-La: studies on sera from women with SLE/PAPS.

Lupus·2007

Same author

Outcome of a national Israeli cohort of pediatric systemic lupus erythematosus.

Lupus·2007

Area of Science:

Immunology
Cell Biology
Autoimmunity

Background:

Programmed cell death (PCD) involves cell killing and subsequent removal by phagocytes.
Normally, phagocytes do not mount inflammatory responses or produce autoantibodies against apoptotic cells.
Apoptotic cells expose self-antigens on their surface, like nucleosomes.

Purpose of the Study:

To investigate if excess or abnormally processed apoptotic cells can induce autoantibody production.
To understand the mechanisms behind autoantigen presentation and immune response.

Main Methods:

Induction of syngeneic apoptotic cell load in normal mice.
Monitoring for hypergammaglobulinemia, specific autoantibodies (anti-DNA, anticardiolipin), and glomerular deposition.
Analysis of apoptotic cell uptake mechanisms, including complement involvement.

Main Results:

Syngeneic apoptotic load induced transient hypergammaglobulinemia and autoantibodies against DNA and cardiolipin in mice.
Glomerular depositions were observed in mice subjected to apoptotic load.
Complement-mediated opsonization is a key mechanism for apoptotic cell uptake.

Conclusions:

Aberrant handling of apoptotic cells, potentially due to complement deficiencies, can lead to immunogenicity.
The immunogenicity of apoptotic cells may explain autoantigen selection in autoimmune diseases like systemic lupus erythematosus (SLE).

Related Experiment Videos