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From long range mapping to sequence-ready contigs on human chromosome 6.

A J Mungall1, S J Humphray, S A Ranby

  • 1The Sanger Centre, Hinxton, Cambridge, UK.

DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1997
PubMed
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This summary is machine-generated.

Researchers are building detailed physical maps of human chromosome 6 using radiation hybrid (RH) mapping to sequence the entire chromosome. This involves creating a high-density marker map and assembling bacterial clones into contigs for DNA sequencing.

Area of Science:

  • Genomics
  • Human Genetics
  • Molecular Biology

Background:

  • Chromosome 6 contains numerous genes crucial for human health.
  • Previous mapping efforts left significant regions of chromosome 6 uncharacterized.
  • A comprehensive physical map is essential for understanding chromosome 6 structure and function.

Purpose of the Study:

  • To construct high-resolution physical clone maps for under-mapped regions of chromosome 6.
  • To generate a complete DNA sequence of human chromosome 6.
  • To facilitate future research into chromosome 6-associated diseases.

Main Methods:

  • Radiation hybrid (RH) mapping to establish a framework map with 15 markers per Megabase.
  • Identification of large-insert bacterial clones covering chromosome 6.

Related Experiment Videos

  • Assembly of clones into sequence-ready contigs using restriction enzyme fingerprinting and STS content analysis.
  • Gap closure via walking experiments using end-sequence derived STSs.

    • Main Results:

    • Development of a high-density framework map for previously unmapped chromosome 6 regions.
    • Assembly of overlapping bacterial clones into contiguous sequences (contigs).
    • Identification of sequence-tagged sites (STSs) for gap closure and map refinement.

    Conclusions:

    • The established physical map provides a foundation for the complete DNA sequencing of chromosome 6.
    • This work significantly advances the understanding of chromosome 6 organization.
    • The generated resources will accelerate research in human genetics and disease.