Related Experiment Video
Updated: Sep 12, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Published on: February 7, 2019
Solution structure and dynamics of the DNA-binding domain of the adipocyte-transcription factor FREAC-11
M J van Dongen1, A Cederberg, P Carlsson
1Department of Structural Chemistry, Pharmacia and Upjohn, Nordenflychtsvägen 62:5, Stockholm, S-11287, Sweden. maria.vandongen@eu.pnu.com
Abstract:
Transcription factors of the forkhead type share a highly conserved DNA-binding domain of about 100 amino acid residues. FREAC-11, expressed in adipocytes, belongs to this class. Here, we report on NMR studies that established the three-dimensional structure of the FREAC-11, DNA-binding domain. Although apparent similarities to the structures of other members within the forkhead family are observed, the structure also reveals some remarkable differences. Along with the complementary dynamics, the data provide insight into the fundamentals of sequence specificity within a highly conserved motif.
Related Concept Videos
Transcription Factors
Conserved Binding Sites
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Cooperative Binding of Transcription Regulators
Eukaryotic Transcription Activators
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Master Transcription Regulators
Cooperative Binding of Transcription Regulators

